Selenium inhibits Staphylococcus aureus-induced inflammation by suppressing the activation of the NF-κB and MAPK signalling pathways in RAW264.7 macrophages

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• 作者：Chong-Liang Bi^a ; ^b ; ¹ ; Heng Wang^a ; ^b ; Yin-Jie Wang^a ; ^b ; Jun Sun^a ; ^b ; Jun-Sheng Dong^a ; ^b ; Xia Meng^a ; ^b ; Jian-Ji Li^a ; ^b ; ^{yzjjli@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：The chemical compounds that were used in this study were ; selenium (PubChem CID ; 6326970) ; ebselen (PubChem CID ; 3194) ; l-glutamine (PubChem CID ; 5961) ; dimethyl sulfoxide (PubChem CID ; 679) ; diethyl pyrocarbonate (PubChem CID ; 3051) ; sodium dodecyl sulphate (PubChem CID ; 3423265) ; tween 20 (PubChem CID ; 443314)
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：5 June 2016
• 年：2016
• 卷：780
• 期：Complete
• 页码：159-165
• 全文大小：1429 K

文摘

Inflammation is the hallmark of Staphylococcus aureus (S. aureus)-induced mastitis. Given the interesting relationship between selenium levels and inflammation, this study aimed to demonstrate that selenium modulated the inflammation reaction by suppressing the nuclear factor kappa B (NF-κB) and mitogen activated protein kinase (MAPK) signalling pathways. RAW264.7 macrophages were treated with three different concentrations (1 μmol/l, 1.5 μmol/l, and 2 μmol/l) of Na₂SeO₃ for 12 h before infection with S. aureus for 6 h, 8 h, and 10 h. The results showed that selenium significantly reduced the mRNA expression levels of tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). Furthermore, the release of TNF-α, IL-1β, and IL-6 was decreased significantly with selenium supplementation. In addition, selenium influenced the NF-κB signalling pathway by suppressing the activation of NF-κB p65 and degradation of inhibitory kappa-B (IκB). Selenium also suppressed extracellular regulated protein kinase (Erk), c-Jun N-terminal kinase (Jnk), and p38 phosphorylation through the MAPK signalling pathway. In conclusion, selenium played an anti-inflammation role in RAW264.7 macrophages infected with S. aureus by suppressing the activation of the NF-κB and MAPK signalling pathways.