Arabinogalactan protein cluster from Jatropha curcas seed embryo contains fasciclin, xylogen and LysM proteins

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• 作者：Maria Sehlbach ; Simone K?nig ; Michael Mormann ; J ; irk Sendker ; Andreas Hensel
• 关键词：AEC ; anion exchange chromatography ; AGP ; arabinogalactan protein ; DP ; degree of polymerization ; FLA ; fasciclin-like arabinogalactans ; GPC ; gel permeation chromatography ; HPAEC-PAD ; high pressure anion exchange chromatography with pulsed-amperometric detec
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：15 October, 2013
• 年：2013
• 卷：98
• 期：1
• 页码：522-531
• 全文大小：2605 K

文摘

An non-GPI-anchored AGP cluster (Y2) was isolated from the seeds of Jatropha curcas L. (Euphorbiaceae) composed of 4.8 % polypeptides (mainly Ala, Ser, Gly, Hyp, Glu) and a carbohydrate moiety composed of Gal, Ara, GlcA, Rha, Man and GlcN. Besides the typical structural features of arabinogalactan proteins, typical N-glycan linker of the complex type (GlcNAc₄Man₃Gal₂Fuc₁Xyl₁) were identified. O-glycosylation occurred mainly via Hyp and to a lesser extent via Thr and Ser. N-glycans from the complex type, carrying at the innermost GlcNAc at position O-3 one ¦Á-Fuc-residue, were also present.

MS analysis of the tryptic digest assigned peptides of three major protein groups: fasciclin-like arabinogalactan proteins, xylogen-like proteins and LysM domain-containing proteins. They could not be separated further and it is indicated that various homologous protein forms co-exist. Histological investigation of J. curcas seeds revealed the presence of AGPs in the vessels of cotyledons and in the procambium ring of the embryo.