Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans

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• 作者：Xianghong Wang^a ; Daiming Jiang^a ; Jingni Shi^b ; Daichang Yang^a ; ^{dyang@whu.edu.cn} ; ^{dyang@oryzogen.com}
• 关键词：Plant-specific glycans ; N-glycosylation ; Rice seed ; Immunogenicity of plant-specific glycans ; Molecular pharming
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：111-121
• 全文大小：1766 K
• 卷排序：242

文摘

We studied the plant-specific glycan type and composition in rice endosperm cell. We analyzed the α-1,3-fucose contents of the glycoprotein produced from rice endosperm and leaf cells, and found that those glycans were much lower in endosperm than that in leaf cells. The expression of FUT8 gene in rice endosperm cell not only introduced human-specific α-1,6-fucose, but also reduced the α-1,3-fucose content. The immunogenicities studies revealed that no significant difference was found in antibody concentration among OsrAAT, mgOsrAAT and hAAT. This work updates the concept of immunogenicity of plant-specific glycans.