Enhanced production of secretory glycoprotein VSTM1-v2 with mouse IgGκ signal peptide in optimized HEK293F transient transfection
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- 作者：Huihui Liu1 ; 2 ; Xiajuan Zou3 ; Ting Li1 ; 2 ; Xiaolin Wang1 ; 2 ; Wanqiong Yuan1 ; 2 ; Yingyu Chen1 ; 2 ; Wenling Han1 ; 2 ; hanwl@bjmu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cytokine ; VSTM1-v2 ; Secretory glycoprotein ; Signal peptide ; Transient gene expression ; TMPP-Ac derivatization
- 刊名：Journal of Bioscience and Bioengineering
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：121
- 期：2
- 页码：133-139
- 全文大小：1367 K
文摘
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Mouse IgGκ signal peptide greatly enhanced secretion capacity of VSTM1-v2.
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The yield of VSTM1-v2 in the optimized suspension HEK293F transient expression system was increased by 7 folds.
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VSTM1-v2 was synthesized and secreted as a hyper-glycosylated protein with complex oligosaccharides.
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The purified VSTM1-v2 exhibited excellent bioactivity.
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The study provides a high-efficient way to rapidly enhance production of proteins with weak secretion capacity.