Click labeling of unnatural sugars metabolically incorporated into viral envelope glycoproteins enables visualization of single particle fusion
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- 作者：Yoon Hyeun Ouma ; 1 ; Tanay M. Desaia ; Mariana Marina ; Gregory B. Melikyana ; b ; gmeliki@emory.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ac4ManNAz ; peracylated azidomannosamine ; ASLV ; Avian Sarcoma and Leukosis Virus ; Env ; viral envelope glycoprotein ; SiaNAz ; N-azidosialic acid ; VSV ; Vesicular Stomatitis Virus ; YFP ; yellow fluorescent protein
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：62-71
- 全文大小：3374 K
文摘
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Unnatural sugar, Ac4ManNAz, can be efficiently incorporated into viral glycoproteins without significantly compromising their functional activity.
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Copper-free click labeling of viral glycoproteins containing unnatural sugars with organic fluorophores does not affect their ability to mediate membrane fusion.
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Click labeling of viral glycoproteins containing unnatural sugars does not affect their ability to mediate membrane fusion.