Guidelines for cloning, expression, purification and functional characterization of primary HIV-1 envelope glycoproteins
详细信息 查看全文
- 作者：Yann Benureaua ; b ; yann.benureau@free.fr" class="auth_mail" title="E-mail the corresponding author ; Philippe Colina ; b ; philippe.colin1@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Isabelle Staropolia ; b ; isabelle.staropoli@pasteur.fr" class="auth_mail" title="E-mail the corresponding author ; Nuria Gonzalezc ; nglez@isciii.es" class="auth_mail" title="E-mail the corresponding author ; Javier Garcia-Perezc ; eoaz@isciii.es" class="auth_mail" title="E-mail the corresponding author ; Jose Alcamic ; ppalcami@isciii.es" class="auth_mail" title="E-mail the corresponding author ; Fernando Arenzana-Seisdedosa ; b ; farenzan@pasteur.fr" class="auth_mail" title="E-mail the corresponding author ; Bernard Laganea ; b ; bernard.lagane@pasteur.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：HIV-1 gp120 ; AIDS ; Cloning ; Gene toxicity ; Protein expression and purification ; Receptor binding assays
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：184-195
- 全文大小：2296 K
文摘
- •
A reliable protocol for producing primary monomeric HIV-1 gp120 is presented.
- •
The protocol avoids renowned problems in amplifying and cloning HIV-1 Env sequences.
- •
The conditions for studying the gp120 interactions with CD4 and CCR5 are described.
- •
The binding assay to CD4 is adaptable to large collections of glycoproteins.
- •
The receptor binding assays confirmed that the gp120 are functionally relevant.