Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803
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- 作者：Artur Wlodarczyka ; 1 ; Thiyagarajan Gnanasekarana ; 1 ; Agnieszka Zygadlo Nielsena ; d ; Nodumo Nokolunga Zulub ; Silas Busck Mellora ; Manja Lucknerc ; Jens Frederik Bang Thø ; fnera ; Carl Erik Olsena ; Mohammed Saddik Mottawiea ; Meike Burowa ; e ; Mathias Pribila ; Ivo Feussnerb ; Birger Lindberg Mø ; llera ; d ; Poul Erik Jensena ; d ; peje@plen.ku.dk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cyanobacteria ; Synechocystis ; Cytochrome P450 ; Metabolic engineering ; Cyanogenic glucosides ; Light-driven biosynthesis
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：33
- 期：Complete
- 页码：1-11
- 全文大小：2339 K
文摘
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Expression of multiple genes using self-replicating expression vector in cyanobacteria
Eukaryotic P450s can be expressed and inserted in cyanobacterial membranes.
Cyanobacterial photosystem I and ferredoxin are efficient electron donors for plant P450s.
Pathway intermediates and final products are excreted to the growth media.
Engineered strain was grown in a photobioreactor using simple nutrient mixture.