Functional activity of the porcine Gnrhr2 gene promoter in testis-derived cells is partially conferred by nuclear factor-κB, specificity protein 1 and 3 (SP1/3) and overlapping early growth response 1/SP1/3 binding sites
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- 作者：Vanessa M. Brauer1 ; Jocelyn R. Wiarda-Bell2 ; Amy T. Desaulniers ; Rebecca A. Cederberg ; Brett R. White ; bwhite2@unl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：GnRH1 ; gonadotropin-releasing hormone 1 ; GnRH2 ; gonadotropin-releasing hormone 2 ; GnRHR1 ; gonadotropin-releasing hormone 1 receptor ; GnRHR2 ; gonadotropin-releasing hormone 2 receptor ; ST ; swine testis-derived ; EMSA ; electrophoretic mobility shift assay ; NF-κB ; nuclear factor-κB ; SP1 ; specificity protein 1 ; SP3 ; specificity protein 3 ; EGR1 ; early growth response 1 ; LH ; luteinizing hormone ; FSH ; follicle stimulating hormone ; SF1 ; steroidogenic factor 1 ; AP-1 ; activator protein-1 ; GRAS ; GnRH recept
- 刊名：Gene
- 出版年：2016
- 出版时间：10 August 2016
- 年：2016
- 卷：587
- 期：2
- 页码：137-146
- 全文大小：919 K
文摘
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The GnRHR2 promoter is active in cell types derived from extra-pituitary tissues.
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Transcriptional regulation of the porcine GnRHR2 gene in the testis was examined.
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A putative repressor at − 1693/− 709 and activator at − 708/− 489 were identified.
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NF-κB, SP1/3 and overlapping EGR1/SP1/3 sites confer activity in the ST cell line.
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This is the first report of functional elements within the GnRHR2 gene promoter.