Influence of nanoparticle diameter on conjugated enzyme activity

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• 作者：Joey N. Talbert ; Julie M. Goddard
• 关键词：Galactosidase ; Lactase ; Nanoparticle ; Nanomaterial ; Bioprocessing ; Immobilized enzyme
• 刊名：Food and Bioproducts Processing
• 出版年：October, 2013
• 年：2013
• 卷：91
• 期：4
• 页码：693-699
• 全文大小：922 K

文摘

Lactase conjugated to nanomaterials represents an area of significant potential to the food processing as a means to produce novel value-added products, reduce waste, and enable diagnostics. While it is recognized that, in general, matter exhibits unique properties when manipulated at the nanoscale, little is known about how reducing the size of the carrier to the nanoscale effects attached lactase. The purpose of this work is to investigate the influence of particle size on activity retention of lactase (m>Aspergillus oryzaem>) covalently conjugated to magnetic nanoparticles of varying sizes. Lactase was attached to carboxylic acid functionalized magnetic nanoparticles 18 nm, 50 nm, and 200 nm in diameter using carbodiimide chemistry. After attachment, activity retention was 73%, 39%, and 14% compared to the free enzyme for the 18 nm, 50 nm, and 200 nm conjugates, respectively. The apparent m>Km>_m was not significantly different as a function of particle size while the apparent m>km>_cat decreased with increasing particle size. Reducing the particle size of magnetic nanoparticles can increase the activity retention of conjugated lactase. This work provides improved understanding of enzyme-nanoparticles systems and allows for enhanced design of lactase-conjugated materials.