Perrin and Förster unified: Dual-laser triple-polarization FRET (3polFRET) for interactions at the Förster-distance and beyond
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- 作者：Tamá ; s Ungvá ; rib ; Pé ; ter Gogolá ; ka ; Mikló ; s Bagdá ; nyc ; Lá ; szló ; Damjanovichd ; Lá ; szló ; Bened ; bene@med.unideb.hu" class="auth_mail" title="E-mail the corresponding author
- 关键词：FRET ; fluorescence resonance energy transfer ; MHCI/MHCII ; Class I/Class II Major Histocompatibility Complex protein ; β2m ; beta-2 microglobulin ; the light chain (l.c.) component of MHCI ; mAb ; monoclonal antibody
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular Cell Research
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1863
- 期：4
- 页码：703-716
- 全文大小：1249 K
文摘
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FCET is extended into the domain of receptor dynamics by the detection of polarized components of the 3 intensities.
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Cell-by-cell distributions of FRET-fraction and rotational characteristics of the donor, and κ2 are available in parallel.
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“Polarized FRET-indices” made from FRET efficiency and anisotropies are introduced for detecting conformational changes.
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The existence of a receptor trimer is shown by decreasing homo-FRET between two donors by an acceptor (homo-FRET relief).
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Monitoring triple-anisotropy correlations also in those cases when FRET actually does not occur, e.g. beyond R0.