Chitosan-based DNA delivery vector targeted to gonadotropin-releasing hormone (GnRH) receptor
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- 作者：Chatwalee Boonthum
- 关键词：Gonadotropin releasing hormone (GnRH) ; Chitosan ; Gene delivery
- 刊名：Carbohydrate Polymers
- 出版年：2017
- 出版时间：10 February 2017
- 年：2017
- 卷：157
- 期：Complete
- 页码：311-320
- 全文大小：2813 K
- 卷排序：157
文摘
The main purpose of this study was to investigate the application of modified chitosan as a potential vector for gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells. Such design of gene carrier could be useful in particular for gene therapy for cancers related to the reproductive system, gene disorders of sexual development, and contraception and fertility control. In this study, a decapeptide GnRH was successfully conjugated to chitosan (CS) as confirmed by proton nuclear magnetic resonance spectroscopy (1H NMR) and Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The synthesized GnRH-conjugated chitosan (GnRH-CS) was able to condense DNA to form positively charged nanoparticles and specifically deliver plasmid DNA to targeted cells in both two-dimensional (2D) and three-dimensional (3D) cell cultures systems. Importantly, GnRH-CS exhibited higher transfection activity compared to unmodified CS. In conclusion, GnRH-conjugated chitosan can be a promising carrier for targeted DNA delivery to GnRHR-expressing cells.