Effects of 1,25-dihydroxyvitamin D₃ on proliferation and differentiation of porcine preadipocyte in vitro

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• 作者：Helin Zhuang ; Yaqiu Lin ; Gongshe Yang
• 关键词：1 ; 25-Dihydroxyvitamin D₃ ; Proliferation ; Differentiation ; Adipogenesis ; Porcine preadipocyte ; In vitro
• 刊名：Chemico-Biological Interactions
• 出版年：2007
• 出版时间：20 November 2007
• 年：2007
• 卷：170
• 期：2
• 页码：114-123
• 全文大小：832 K

文摘

1,25-Dihydroxyvitamin D₃, the physiologically active form of vitamin D₃, exerts its functions through a receptor-mediated mechanism and plays an important role in the cell differentiation. This study investigated the effects of 1,25-dihydroxyvitamin D₃ on the proliferation and differentiation of porcine preadipocyte. Stromal-vascular cells containing preadipocytes were prepared from dorsal subcutaneous adipose tissue of approximately 3-day-old Chinese male crossbred pigs. After confluence, the differentiation was induced by transferrin, dexamethasone and insulin for 2 days, and then subsequently cultured for 6 days. The cells were treated with 1,25-dihydroxyvitamin D₃ during the induction of differentiation (the early phase of differentiation) or throughout the differentiation period. The terminal differentiation markers, such as glycerol-3-phosphate dehydrogenase activity and lipid accumulation were measured during the process of cultures. The treatment with 1,25-dihydroxyvitamin D₃ severely affected the induction of all differentiation markers throughout the differentiation period. 1,25-Dihydroxyvitamin D₃ suppressed the expression of peroxisome proliferator-activated receptor gamma mRNA and interfered with the induction of retinoid X receptor alpha mRNA. The mRNAs of the adipogenesis-related genes, lipoprotein lipase, stearoyl-CoA desaturase, phosphoenolpyruvate carboxykinase, glycerol-3-phosphate dehydrogenase and glucose transporter 4 were reduced when 1,25-dihydroxyvitamin D₃ was added into differentiation medium. Also, 1,25-dihydroxyvitamin D₃ inhibited preadipocyte differentiation in dose-dependent manner. These results suggested that 1,25-dihydroxyvitamin D₃ inhibited porcine preadiopocyte differentiation through suppressing PPARγ and RXRα mRNA expressions and then down regulating the expression of adipogenesis-related genes.