NXF-2, REF-1, and REF-2 Affect the Choice of Nuclear Export Pathway for tra-2 mRNA in C. elegans

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• 作者：Scott Kuersten ; Scott P. Segal ; Jamie Verheyden ; Sarah M. LaMartina and Elizabeth B. Goodwin
• 刊名：Molecular Cell
• 出版年：2004
• 出版时间：4 June 2004
• 年：2004
• 卷：14
• 期：5
• 页码：599-610
• 全文大小：567 K

文摘

In C. elegans, tra-2 mRNA nuclear export is controlled by a 3′UTR element, the TRE. In the absence of TRA-1, the TRE retains tra-2 mRNA in the nucleus. The binding of TRA-1 to the 3′UTR overcomes this retention resulting in export of a TRA-1/tra-2 mRNA complex. Here, we find that, unlike most mRNAs, tra-2 mRNA exits the nucleus via an alternative pathway to NXF-1 that requires CRM1 activity. Inhibition of export by NXF-1 depends upon the TRE, CeNXF-2, CeREF-1, and CeREF-2. Removal of the TRE or any one of these factors results in export of tra-2 by NXF-1. NXF-2 and REF-1 specifically bind the TRE, suggesting that they directly control tra-2 mRNA export. Furthermore, choice of proper export pathway affects tra-2 translational control. Therefore, tra-2 mRNA export is highly regulated and plays an important role in development by regulating the activity of tra-2 mRNA in the cytoplasm.