Cancer cell detection in tissue sections using AFM

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• 作者：Mał ; gorzata Lekka^a ; ^{Malgorzata.Lekka@ifj.edu.pl} ; Dorota Gil^b ; Katarzyna Pogoda^a ; Joanna Duliń ; ska-Litewka^b ; Robert Jach^c ; Justyna Gostek^a ; Olesya Klymenko^a ; Szymon Prauzner-Bechcicki^a ; Zbigniew Stachura^a ; Joanna Wiltowska-Zuber^a ; Krzysztof Okoń ; ^d ; Piotr Laidler^b
• 关键词：Single cell biomechanics ; Tissue stiffness ; Atomic force microscopy ; Cancer
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2012
• 出版时间：15 February 2012
• 年：2012
• 卷：518
• 期：2
• 页码：151-156
• 全文大小：1096 K

文摘

Currently, cancer diagnosis relies mostly on morphological examination of exfoliated, aspirated cells or surgically removed tissue. As long as standard diagnosis is concerned, this classical approach seems to be satisfactory. In the recent years, cancer progression has been shown to be accompanied by alterations in mechanical properties of cells. This offers the detection of otherwise unnoticed cancer cell disregarded by histological analysis due to insignificant manifestations. One of techniques, sensitive to changes in mechanical properties, is the atomic force microscopy, which detects cancer cells through their elastic properties. Such measurements were applied to tissue sections collected from patients suffering from various cancers. Despite of heterogeneity and complexity of cancer cell sections, the use of the Young¡¯s modulus as an indicator of cell elasticity allow for detection of cancer cells in tissue slices.