- 作者：Sylvia Vogl ; Paolo Picker ; Judit Mihaly-Bison ; Nanang Fakhrudin ; Atanas G. Atanasov ; Elke H. Heiss ; Christoph Wawrosch ; Gottfried Reznicek ; Verena M. Dirsch ; Johannes Saukel ; Brigitte Kopp
- 关键词：Traditional European medicine (TEM) ; Austria ; Inflammation ; PPAR ; NF-?B ; IL-8 ; E-selectin
- 刊名：Journal of Ethnopharmacology
- 出版年：2013
- 出版时间：7 October, 2013
- 年：2013
- 卷：149
- 期：3
- 页码：750-771
- 全文大小：644 K
Ethnopharmacological relevance
In Austria, like in most Western countries, knowledge about traditional medicinal plants is becoming scarce. Searching the literature concerning Austria's ethnomedicine reveals its scant scientific exploration.Aiming to substantiate the potential of medicinal plants traditionally used in Austria, 63 plant species or genera with claimed anti-inflammatory properties listed in the VOLKSMED database were assessed for their in vitro anti-inflammatory activity.
Material and methods
71 herbal drugs from 63 plant species or genera were extracted using solvents of varying polarities and subsequently depleted from the bulk constituents, chlorophylls and tannins to avoid possible interferences with the assays. The obtained 257 extracts were assessed for their in vitro anti-inflammatory activity. The expression of the inflammatory mediators E-selectin and interleukin-8 (IL-8), induced by the inflammatory stimuli tumor necrosis factor alpha (TNF-¦Á) and the bacterial product lipopolysaccharide (LPS) was measured in endothelial cells. The potential of the extracts to activate the nuclear factors PPAR¦Á and PPAR¦Ã and to inhibit TNF-¦Á-induced activation of the nuclear factor-kappa B (NF-¦ÊB) in HEK293 cells was determined by luciferase reporter gene assays.
Results
In total, extracts from 67 of the 71 assessed herbal drugs revealed anti-inflammatory activity in the applied in vitro test systems. Thereby, 30 could downregulate E-selectin or IL-8 gene expression, 28 were strong activators of PPAR¦Á or PPAR¦Ã (inducing activation of more than 2-fold at a concentration of 10 ¦Ìg/mL) and 21 evoked a strong inhibition of NF-¦ÊB (inhibition of more than 80 % at 10 ¦Ìg/mL).
Conclusion
Our research supports the efficacy of herbal drugs reported in Austrian folk medicine used for ailments associated with inflammatory processes. Hence, an ethnopharmacological screening approach is a useful tool for the discovery of new drug leads.