Monocyte-derived cells express CYP27A1 and convert vitamin D3 into its active metabolite

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• 作者：Eva Gottfried ; Michael Rehli ; Joachim Hahn ; Ernst Holler ; Reinhard Andreesen and Marina Kreutz
• 关键词：Monocyte ; Macrophage ; Dendritic cell ; Active vitamin D3 ; CYP27A1 ; CYP27B1 ; CYP24A1
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：13 October 2006
• 年：2006
• 卷：349
• 期：1
• 页码：209-213
• 全文大小：149 K

文摘

CYP27A1 catalyses hydroxylations in the biosynthesis of bile acids and the bioactivation of vitamin D3. We investigated the expression of CYP27A1 in human monocytes, monocyte-derived macrophages, and dendritic cells on mRNA and protein levels as well as its enzymatic activity in comparison with the expression of CYP27B1 and CYP24A1. Macrophages showed a strong expression of CYP27A1, whereas monocytes and dendritic cells expressed low levels of CYP27A1 mRNA. Immunohistochemistry revealed CYP27A1 and CYP27B1 protein expression in macrophages. Accordingly, macrophages converted vitamin D3 into the active metabolite 1,25(OH)₂D₃. Dendritic cells also metabolized vitamin D3 although to a lesser extent. This could be due to the high expression of CYP24A1, the enzyme that degrades 25(OH)D₃ and 1,25(OH)₂D₃. Our results show that macrophages and dendritic cells are capable to perform both hydroxylation steps of the vitamin D3 metabolism suggesting a possible role of local 1,25(OH)₂D₃ synthesis by myeloid cells in the skin and gut.