Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

详细信息    查看全文

• 作者：Mohan C. Manjegowda ; Paridhi Singhal Gupta ; Anil M. Limaye ; ^{amul@iitg.ernet.in" class="auth_mail" title="E-mail the corresponding author}
• 关键词：GPR30 ; Antiserum ; Affinity purified antibody ; Western blotting
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：1015-1020
• 全文大小：654 K

文摘

Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines.