Systemic identification of estrogen-regulated genes in breast cancer cells through cap analysis of gene expression mapping
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- 作者：Ryonosuke Yamagaa ; b ; c ; Kazuhiro Ikedaa ; Joost Boeled ; g ; Kuniko Horie-Inouea ; Ken-ichi Takayamab ; c ; Tomohiko Uranob ; c ; Kaoru Kaidae ; g ; Piero Carnincie ; g ; Jun Kawaif ; g ; Yoshihide Hayashizakif ; g ; Yasuyoshi Ouchib ; Michiel de Hoone ; g ; Satoshi Inouea ; b ; c ; INOUE-GER@h.u-tokyo.ac.jp" class="auth_mail
- 关键词：Estrogen ; Breast cancer ; Cap analysis of gene expression ; Transcriptome
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：9 May 2014
- 年：2014
- 卷：447
- 期：3
- 页码：531-536
- 全文大小：507 K
文摘
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Cap analysis of gene expression sequencing is performed in breast cancer MCF-7 cells.
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The method identifies 15 CAGE tag clusters close to estrogen receptor binding sites.
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RAMP3, ISOC1 and GPRC5C are estrogen-regulated genes with those CAGE tag clusters.
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RAMP3, ISOC1 and GPRC5C will regulate growth/migration of MCF-7 cells.
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CAGE sequencing is useful for global identification of estrogen-regulated promoters.