Accurate fat fraction quantification by multiecho gradient-recalled-echo magnetic resonance at 1.5 T in rats with nonalcoholic fatty liver disease

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• 作者：Elizabeth Hijona^a ; ^{eli.hijonamuruamendiaraz@osakidetza.net} ; Javier Sá ; nchez-Gonzá ; lez^b ; Jose M. Alú ; stiza^c ; Lander Hijona^d ; Juan Arenas^a ; Elisabeth Garcí ; a^c ; Nohelia Rojas^a ; Maria P. Portillo^e ; Raú ; l Jimé ; nez^a ; Pablo Aldazabal^a ; Luis Bujanda^a
• 关键词：Fatty liver ; Magnetic resonance ; Fat ; multi-echo ; Biochemical ; Rats
• 刊名：European Journal of Radiology
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：81
• 期：6
• 页码：1122-1127
• 全文大小：658 K

文摘

Aim

To assess the diagnostic accuracy of a new reconstruction technique for gradient-recalled-echo magnetic resonance (MR) sequences that provides a full decomposition of the water and fat content inside a voxel for nonalcoholic fatty liver disease (NAFLD) in rats.

Material and methods

Rats were randomized into two groups. A control group (n = 10) was given free access to regular dry rat chow for 4 weeks. The steatosis (n = 40) group was given free access to feed and water 4 days per week, and fasted for the remaining 3 days for 4 weeks. All rats were killed at 4 weeks and assessed for fatty infiltration and biochemical method.

Results

The average fat content using the gold standard method was 2.65 g (2.20-3.05) of fat/100 g liver for the control group and 4.14 g (1.95-8.60) of fat/100 g of liver for the overfed group (p < 0.05). The average fat-fraction obtained from the MR was 0.016 (0.01-0.02) for the control group and 0.057 (0.00-0.18) for the overfed group. The Pearson correlation coefficient between the samples was r² = 0.87.

Conclusion

Multi-echo MR is a good technique to quantify liver fat in rats.