Identification of voltage-dependent Cap>2+ p> channels in sea urchin sperm
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- 作者：G. Granados-Gonzalez ; I. Mendoza-Lujambio ; E. Rodriguez ; B.E. Galindo ; C. Beltrá ; n ; A. Darszon
- 关键词：Speract ; Calcium channels ; Acrosome reaction ; Motility
- 刊名：FEBS Letters
- 出版年：2005
- 出版时间：5 December 2005
- 年：2005
- 卷：579
- 期：29
- 页码：6667-6672
- 全文大小：262 K
文摘
Functional evidence indicates that voltage-dependent Cap>2+ p> (Cav) channels participate in sea urchin sperm motility and the acrosome reaction (AR), however, their molecular identity remains unknown. We have identified transcripts for two Cap>2+ p> channel α1 subunits in sea urchin testis similar in sequence to Cav1.2 and Cav2.3. Antibodies against rat Cav1.2 and Cav2.3 channels differentially label proteins in the flagella and acrosome of mature sea urchin sperm. The Cav channel antagonists nifedipine and nimodipine, which inhibit the AR, diminish the intracellular Cap>2+ p> elevation induced by a Kp>+ p>-induced depolarization in valinomycin-treated sperm. These findings reveal that Cav1.2 and Cav2.3 channels could participate in motility and/or the AR in sea urchin sperm.