Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability

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• 作者：Lena Rydzewski^a ; ^{Lena.rydzewski@vetmed.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author} ; Svenja Scheffold^a ; Werner Hecht^a ; Eberhard Burkhardt^a ; Katharina Kerner^b ; Michele C. Klymiuk^c ; Renate Deinzer^d ; Manfred Reinacher^a ; Manfred Henrich^a
• 关键词：Centrosome amplification ; Cytotoxic T cells ; Genetic instability ; Large granular lymphocyte ; Lymphoma ; p53
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：177
• 期：Complete
• 页码：24-34
• 全文大小：4438 K

文摘

Feline large granular lymphocyte lymphomas are rare but very aggressive tumors with a poor prognosis. In this study, a cell line from an abdominal effusion of a cat with large granular lymphoma was characterized. Immunophenotype staining was positive for CD3 and CD45R, and negative for CD4, CD8, CD56, CD79α, BLA.36 and NK1. A TCR γ gene rearrangement was detectable by PARR. Neither FeLV antigen nor exogenous FeLV provirus could be detected. A chromosomal instability associated with a centrosome hyperamplification could also be determined. The cell line is able to lyse target cells without antigen presentation or interaction with antigen presenting cells. Therefore, these cells were classified as genetically instable non-MHC-restricted cytotoxic T cells with large granular lymphocyte morphology.