Clonal analysis of human dendritic cell progenitor using a stromal cell culture

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• 作者：Jaeyop Lee^a ; Gaë ; lle Breton^b ; Arafat Aljoufi^a ; Yu Jerry Zhou^a ; Sarah Puhr^a ; Michel C. Nussenzweig^b ; ^c ; Kang Liu^a ; ^{kl2529@columbia.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Hematopoiesis ; Dendritic cell ; Single cell ; Culture ; Granulocyte&ndash ; macrophage progenitor ; Stromal cells
• 刊名：Journal of Immunological Methods
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：425
• 期：Complete
• 页码：21-26
• 全文大小：1042 K

文摘

Different dendritic cell (DC) subsets co-exist in humans and coordinate the immune response. Having a short life, DCs must be constantly replenished from their progenitors in the bone marrow through hematopoiesis. Identification of a DC-restricted progenitor in mouse has improved our understanding of how DC lineage diverges from myeloid and lymphoid lineages. However, identification of the DC-restricted progenitor in humans has not been possible because a system that simultaneously nurtures differentiation of human DCs, myeloid and lymphoid cells, is lacking. Here we report a cytokine and stromal cell culture that allows evaluation of CD34⁺ progenitor potential to all three DC subsets as well as other myeloid and lymphoid cells, at a single cell level. Using this system, we show that human granulocyte–macrophage progenitors are heterogeneous and contain restricted progenitors to DCs.