Increased choline uptake in macrophages and prostate cancer cells does not allow for differentiation between benign and malignant prostate pathologies

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• 作者：Timo Schwarz^a ; ^b ; Christof Seidl^a ; ^c ; ^{christof.seidl@lrz.tum.de" class="auth_mail" title="E-mail the corresponding author} ; Matthias Schiemann^d ; Reingard Senekowitsch-Schmidtke^a ; Bernd Joachim Krause^a ; ^e
• 关键词：[3H]choline ; [18F]FDG ; PC-3 cells ; LNCaP cells ; Leucocytes ; Inflammatory cells
• 刊名：Nuclear Medicine and Biology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：43
• 期：6
• 页码：355-359
• 全文大小：403 K

文摘

Inflammatory cells may contribute to the choline uptake in different prostate pathologies. The aim of this study was (i) to assess if inflammatory cells incorporate choline and (ii) to potentially detect differences compared to FDG uptake. Therefore we investigated the uptake of [³H]choline and [¹⁸F]FDG in human prostate carcinoma cells and human inflammatory cells.

Methods

Macrophages were cultured from isolated mononuclear cells, gained by density gradient centrifugation of human buffy coats. T-lymphocytes, B-lymphocytes and granulocytes were enriched by density gradient centrifugation before cell sorting by means of flow cytometry was performed. [³H]choline and [¹⁸F]FDG uptake of isolated inflammatory cells as well as of LNCaP and PC-3 human prostate carcinoma cells was assessed simultaneously in dual tracer uptake experiments.

Results

Macrophages showed highest [³H]choline and [¹⁸F]FDG uptake compared to the tracer uptake rates of leukocytes. [³H]choline uptake of macrophages was in the same range as in prostate cancer cells. Lipopolysaccharide stimulation of macrophages resulted in an increase of [¹⁸F]FDG uptake in macrophages, but not in an increased [³H]choline uptake.

Conclusions

The high [³H]choline uptake in macrophages may be a source of false-positive PET results in diagnosis of prostate cancer by choline-PET/CT. As already known from FDG-PET, discrimination between tumor and inflammation in prostate cancer patients is not possible via choline-PET.

Advances in knowledge and implications for patient care

The application of choline-PET for reliable primary prostate cancer detection and delineation has to be queried.