Field applications of improved formulations of Candida sake CPA-1 for control of Botrytis cinerea in grapes
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- 作者：Teresa Paula Cañ ; amá ; s ; Immaculada Viñ ; as ; Rosario Torres ; Josep Usall ; Cristina Solsona ; Neus Teixidó
- 关键词：Candida sake ; Fungicover® ; Grapevine ; Biological control ; Biological formulations ; Efficacy ; Persistence ; Survival
- 刊名：Biological Control
- 出版年：2011
- 出版时间：February 2011
- 年：2011
- 卷：56
- 期：2
- 页码：150-158
- 全文大小：1191 K
文摘
There is increasing interest in the use of biological control agents (BCAs) to replace synthetic fungicides. The aim of this study was to evaluate the effectiveness of combining different formulation strategies on the establishment, survival, persistence and efficacy of Candida sake CPA-1 applied in the field in order to control Botrytis cinerea in grapes. Different formulations of C. sake cells were applied at flowering, pea sized berries, veraison and before harvest. In 2005, heat adapted or non-adapted fresh cells of C. sake were tested. In 2006, these same treatments were tested but, in this case, the C. sake cells were also formulated in an isotonic solution. The compound Fungicover® (FC) an edible coating was evaluated as a potential additive for C. sake treatments. Spray applications of different formulations of C. sake resulted in colonisation of bunches under field conditions and when combined with FC, C. sake had significantly higher survival rates (up to 50 % higher) compared with C. sake without additive FC. Formulation of C. sake cells in an isotonic solution combined with FC resulted in Botrytis spp. control similarly than fungicide treatment. The potential of C. sake for biocontrol of Botrytis bunch rot of grapevine has been demonstrated. FC has a beneficial effect on the BCA, improving the persistence of C. sake cells on the host and its efficacy to levels comparable to fungicide treatment. It is possible to broaden the spectrum of use of BCAs using different formulation strategies and to thereby develop practical uses under field conditions.