DNA damage in organs of mice treated acutely with patulin, a known mycotoxin
详细信息 查看全文
- 作者：Fl¨¢via Terezinha de Melo ; Iuri Marques de Oliveira ; Samuel Greggio ; Jaderson Costa Dacosta ; Temenouga Nikolova Guecheva ; Jenifer Saffi ; Jo?o Antonio P¨ºgas Henriques ; Renato Moreira Rosa
- 关键词：DTNB ; 5 ; 5&prime ; -dithionitrobenzoic acid ; GSH ; reduced glutathione ; GSSG ; oxidized glutathione ; HBSS ; Hank&rsquo ; s balanced salt solution ; MDA ; malondialdehyde ; MMS ; methyl methanesulphonate ; N-ac ; N-acetyl-cysteine ; NADPH ; glutathione reductase ; PBS ; pho
- 刊名：Food and Chemical Toxicology
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：50
- 期：10
- 页码：3548-3555
- 全文大小：268 K
文摘
Patulin, a known mycotoxin, is considered a significant contaminant in apples, apple-derived products and feeds. This study investigated the genotoxic effects of patulin in multiple organs (brain, kidney, liver and urinary bladder) of mice using an in vivo comet assay. We assessed the mechanism underlying this genotoxicity by measuring the GSH content and the thiobarbituric acid-reactive species (TBARS) level. Male CF-1 mice were given 1.0-3.75 mg/kg patulin intraperitoneally. The effect of patulin was dose-dependent and the highest patulin dose induced DNA strand breaks in the brain (damage index, DI, in hippocampus increased from 36.2 in control animals to 127.5), liver (44.3-138.4) and kidneys (31.5-99); decreased levels of GSH (hippocampus - from 46.9 to 18.4 nmol/mg protein); and an increase in lipid peroxidation (hippocampus - from 5.8 to 20.3 MDA equivalents/mg protein). This finding establishes an interrelationship between the pro-oxidant and genotoxic effects of patulin. Pre-treatment administration of N-acetyl-cysteine reduced patulin-induced DNA damage (hippocampus - DI from 127.5 to 39.8) and lipid peroxidation (hippocampus - 20.3 to 12.8 MDA equivalents/mg protein) by restoring cellular GSH levels, reinforcing the positive relationship between patulin-induced GSH depletion and DNA damage caused by systemic administration of this mycotoxin.