Identification of major metal complexing compounds in Blepharis aspera
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- 作者：Edward Eddie Mmatli ; Helle Malerø ; d ; Steven Ray Wilson ; Berhanu Abegaz ; Tyge Greibrokk ; Elsa Lundanes ; Karl E. Malterud ; Dirk Petersen ; Frode Rise
- 关键词：Blepharis aspera ; Botswana ; Liquid chromatography– ; nuclear magnetic resonance ; Verbascoside ; Isoverbascoside
- 刊名：Analytica Chimica Acta
- 出版年：2007
- 出版时间：30 July 2007
- 年：2007
- 卷：597
- 期：1
- 页码：24-31
- 全文大小：673 K
文摘
Verbascoside and isoverbascoside, present at 0.7 % and 0.2 % (w/w dryweight), were identified to be major compounds that could contribute to the metal complexation in Blepharis aspera collected in Botswana, Africa. The metallophyte B. aspera has high ability to cope with a high level of metal accumulation. The presence of metal complexing compounds and/or antioxidants can prevent oxidative reactions in lipids, proteins and DNA that take place due to the metal accumulation. On-line liquid chromatography–solid phase extraction–nuclear magnetic resonance (LC–SPE–NMR) was applied for the identification, while electrospray-mass spectrometry (ESI-MS) and UV-vis spectroscopy was used to assess whether these compounds can complex with metals. It was found that verbascoside and isoverbascoside may form complexes with nickel, iron (verbascoside only) and copper. Thus, the presence of verbascoside and isoverbascoside can explain the survival of B. aspera in mineral-rich areas.