Comparison of 3 type VII collagen (C7) assays for serologic diagnosis of epidermolysis bullosa acquisita (EBA)

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• 作者：Vannina Seta^a ; Franç ; oise Aucouturier ; PhD^b ; Jonathan Bonnefoy ; PhD^c ; ^d ; Christelle Le Roux-Villet ; MD^a ; Valé ; rie Pendaries ; PhD^e ; Marina Alexandre ; MD^a ; Sabine Grootenboer-Mignot ; MD ; PhD^f ; Michel Heller ; PhD^g ; Nicole Liè ; vre^g ; Liliane Laroche ; MD ; PhD^a ; Fré ; dé ; ric Caux ; MD ; PhD^a ; Matthias Titeux ; PhD^c ; ^d ; Alain Hovnanian ; MD ; PhD^c ; ^d ; ^h ; Catherine Prost-Squarcioni ; MD ; PhD^a ; ^g ; ⁱ ; ^{catherine.prost@aphp.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：autoimmune bullous disease ; biochip ; enzyme-linked immunosorbent assay ; epidermolysis bullosa acquisita ; indirect immunofluorescence ; sensitivity ; serologic diagnosis ; type VII collagen
• 刊名：Journal of the American Academy of Dermatology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：74
• 期：6
• 页码：1166-1172
• 全文大小：575 K

文摘

Serologic diagnosis of epidermolysis bullosa acquisita (EBA) relies on the detection of circulating autoantibodies to type VII collagen (C7).

Objective

We sought to compare the diagnostic performances of a commercialized enzyme-linked immunosorbent assay (ELISA) using C7 noncollagenous (NC) domains (C7-NC1/NC2 ELISA) and indirect immunofluorescence (IIF) biochip test on NC1-C7-expressing transfected cells (IIFT), with a full-length–C7 ELISA developed in our laboratory.

Methods

C7-NC1/NC2 ELISA, IIFT, and full-length–C7 ELISA were run on 77 nonselected consecutive EBA sera.

Results

C7-NC1/NC2 ELISA, IIFT, and full-length–C7 ELISA were positive, respectively, for: 30%, 27%, and 65% of the 77 sera; 43%, 32%, and 80% of 44 sera labeling the salt-split-skin (SSS) floor (F) by IIF (SSS/F⁺); 9%, 22%, and 47% of 32 SSS/F⁻ sera; 28%, 28%, and 58% of classic EBA; 41%, 41%, and 82% of inflammatory EBA; and 18%, 0%, and 55% of mucous-membrane-predominant EBA. Significant differences for all sera were found between: the 2 ELISAs for the 77 sera, SSS/F⁺ and SSS/F⁻ sera, and IIFT versus full-length–C7 ELISA.

Limitations

The retrospective design was a limitation.

Conclusion

C7-NC1/NC2 ELISA and IIFT sensitivities for serologic diagnoses of EBA were low. Full-length–C7 ELISA was significantly more sensitive and could serve as a reference test.