De novo Synthesis and Assembly of rRNA into Ribosomal Subunits during Cold Acclimation in Escherichia coli
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- 作者：Lolita Piersimoni1 ; Mara Giangrossi2 ; Paolo Marchi ; Anna Brandi2 ; Claudio O. Gualerzi ; claudio.gualerzi@unicam.it" class="auth_mail" title="E-mail the corresponding author ; Cynthia L. Pon
- 关键词：IFs ; initiation factors ; RNAP ; RNA polymerase
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：24 April 2016
- 年：2016
- 卷：428
- 期：8
- 页码：1558-1573
- 全文大小：1258 K
文摘
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rRNA transcription and assembly were analyzed during cold adaptation.
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After cold shock, 16S and 23S rRNA transcription and maturation continue at slower rate.
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rRNA molecules are synthesized de novo from all rrn operons of E. coli, mainly from P2 promoters.
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rRNA newly transcribed during cold acclimation is preferentially found in polysomes.
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Ribosomes are slowly assembled during cold adaptation.