A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system
详细信息 查看全文
- 作者：Anna Maria Frassanito ; Laura Barsanti ; Vincenzo Passarelli ; Valtere Evangelista ; Paolo Gualtieri
- 关键词：Rhodopsin-like protein ; Cyanophora paradoxa ; CpR2 ; Cell-free expression ; Absorption spectrum
- 刊名：Journal of Photochemistry and Photobiology B: Biology
- 出版年：2013
- 出版时间：5 August, 2013
- 年：2013
- 卷：125
- 期：Complete
- 页码：188-193
- 全文大小：1509 K
文摘
Here we report the identification and expression of a second rhodopsin-like protein in the alga Cyanophora paradoxa (Glaucophyta), named Cyanophopsin_2. This new protein was identified due to a serendipity event, since the RACE reaction performed to complete the sequence of Cyanophopsin_1, (the first rhodopsin-like protein of C. paradoxa identified in 2009 by our group), amplified a 619 bp sequence corresponding to a portion of a new gene of the same protein family. The full sequence consists of 1175 bp consisting of 849 bp coding DNA sequence and 4 introns of 326 bp. The protein is characterized by an N-terminal region of 47 amino acids, followed by a region with 7 ¦Á-helices of 213 amino acids and a C-terminal region of 22 amino acids. This protein showed high identity with Cyanophopsin_1 and other rhodopsin-like proteins of Archea, Bacteria, Fungi and Algae. Cyanophosin_2 (CpR2) was expressed in a cell-free expression system, and characterized by means of absorption spectroscopy.