Fe3O4@SiO2-Chitosan/GO adsorbent (MSCG) was successfully prepared. MSCG exerts enhanced interaction with the tetracycline (TC). Well explained the role of Cu(II) as the bridge between TC and MSCG. Magnetic separation technology was used to separate MSCG with TC from aqueous solution. The regenerated MSCG exhibited excellent reusability for the adsorption of TC with and without Cu(II).