Quinone-mediated microbial synthesis of reduced graphene oxide with peroxidase-like activity

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• 作者：Guangfei Liu ; Xin Zhang ; Jiti Zhou ; Aijie Wang ; Jing Wang ; Ruofei Jin ; Hong Lv
• 关键词：Shewanella oneidensis ; Mediated reduction ; Graphene ; Peroxidase ; Glucose detection
• 刊名：Bioresource Technology
• 出版年：December, 2013
• 年：2013
• 卷：149
• 期：Complete
• 页码：503-508
• 全文大小：565 K

文摘

The effects of different quinones on graphene oxide (GO) reduction by Shewanella oneidensis MR-1 and the peroxidase activity of the resultant reduced graphene oxide (QRGO) were studied. The presence of 100 渭M anthraquinone-2-sulfonate (AQS), anthraquinone-2,6-disulfonate and 5-hydroxy-1,4-naphthoquinone could lead to 1.6-2.8-fold increase in GO reduction rate, whereas anthraquinone-2-carboxylate slowed down the reduction. The stimulating effects of AQS increased with the increase of its concentration (10-100 渭M). The mediated effects were proved by direct GO reduction by microbially reduced AQS. The mediated reduction of GO to QRGO was characterized by UV-vis, XRD, FTIR, Raman spectra, XPS, TEM and AFM, respectively. The as-prepared QRGO possessed peroxidase-like activity, which could catalyze the oxidation of 3,3鈥?,5鈥?tetramethylbenzidine by H₂O₂, and followed Michealis-Menten kinetics. A colorimetric sensor for quantitative determination of glucose based on the peroxidase activity of QRGO was developed over a range of 1-120 渭M with a detection limit of 1 渭M.