Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds
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- 作者：Xin Chenga ; Weiling Hea ; Feng Huangb ; Ming Huanga ; mhuang@njau.edu.cn" class="auth_mail ; Guanghong Zhoua
- 关键词：Food authentication ; Multiplex real-time PCR ; DNA quantification ; Blood curds
- 刊名：Food Research International
- 出版年：June 2014
- 年：2014
- 卷：60
- 期：Complete
- 页码：30-37
- 全文大小：1141 K
文摘
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We determined TIANamp® Genomic DNA Kit (Tiangen Biotech) to extract DNA from blood curd.
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The limit of detection (LOD) of the assay was 0.15 ng for each target species.
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The sensitivity of the method was 1% for each species analyzed.
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Simultaneous species identification was achieved with greater sensitivity.
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The assay could be applied in practice and contribute to the food authenticity.