Monitoring in real time the cytotoxic effect of Clostridium difficile upon the intestinal epithelial cell line HT29
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- 作者：Lorena Valdé ; s ; Miguel Gueimonde ; Patricia Ruas-Madiedo ; ruas-madiedo@ipla.csic.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Clostridium difficile ; HT29 ; Toxins ; RTCA ; RT-CES ; xCELLigence
- 刊名：Journal of Microbiological Methods
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：119
- 期：Complete
- 页码：66-73
- 全文大小：1177 K
文摘
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The impedance-based RTCA allows monitoring cytotoxicity of C. difficile.
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The release of cytotoxic C. difficile toxins is modified by the culture medium.
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A dose- and time-dependent toxic effect was detected upon HT29 and Caco2 cells.
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A low EC50 upon HT29 was detected for C. difficile producing A and B toxins.
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Decrease in RTCA signal correlates with a cell-rounding image visualization.