Fatty acid shifts and metabolic activity changes of Schizochytrium sp. S31 cultured on glycerol

详细信息    查看全文

• 作者：Guifang Chang ; Zhenglin Luo ; Sitian Gu ; Qinghang Wu ; Ming Chang ; Xingguo Wang
• 关键词：DHA ; Schizochytrium sp. ; Fatty acid shift ; Glycerol dissimilation ; Lipid turnover
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：142
• 期：Complete
• 页码：255-260
• 全文大小：439 K

文摘

DHA production by Schizochytrium sp. S31 was studied in batch cultures on glycerol with stepwise dissolved oxygen strategy. Three growth stages were identified as cell growth, lipid accumulation and lipid turnover. It was revealed that fatty acid (FA) shifts during the three growth stages involved the activity changes of glycerol kinase (GK), FAD⁺-dependent glycerol-3-phosphate dehydrogenase (FAD⁺-G-3-PDH), malic enzyme (ME), ATP citrate lyase (ACL) and NAD⁺-dependent isocitrate dehydrogenase (NAD⁺-ICDH). Glycerol dissimilation in Schizochytrium sp. S31 was suggested via a phosphorylation by GK and a following oxidation by FAD⁺-G-3-PDH. Lipid accumulation of this strain was a growth-associated process, but the assimilable nitrogen depletion enhanced the accumulation of lipids. The exhaustion of glycerol induced the lipid turnover stage, where the short chain fatty acids were preferentially degraded and converted into lipid-free biomass (X_f) which was correlated to the increase of DHA content in biomass.