Improvement of docosahexaenoic acid production on glycerol by Schizochytrium sp. S31 with constantly high oxygen transfer coefficient

详细信息    查看全文

• 作者：Guifang Chang ; Nisi Gao ; Guiwei Tian ; Qinghang Wu ; Ming Chang ; Xingguo Wang
• 关键词：DHA ; Schizochytrium sp. ; Oxygen ; kLa ; Glycerol
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：142
• 期：Complete
• 页码：400-406
• 全文大小：749 K

文摘

Volumetric mass transfer coefficient (k_La) is a key fermentation parameter for the production of docosahexaenoic acid (DHA) from glycerol by Schizochytrium sp. S31. In order to elucidate the effects of k_La on the fermentations, both baffled and unbaffled flask cultures and fed-batch cultures were developed in present work. The results showed that high k_La could effectively increase the DHA concentration, DHA productivity and conversion yield (Y_x/s, g/g). When k_La was set at 1802 ¡À 105 h^?1 in the fed-batch culture, DHA concentration was achieved at 28.93 g/L, DHA productivity at 301 mg/L/h and Y_x/s at 0.44 ¡À 0.02 g/g, all of which were significantly higher than those in the previous similar studies.