One single HPLC-PDA/(-)ESI-MS/MS analysis to simultaneously determine 30 components of the aqueous extract of Rabdosia rubescens

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• 作者：Jingcheng Tang ; Ming Zhao ; ^{mzhao@bjmu.edu.cn} ; Yuji Wang ; Guifeng Kang ; Jianhui Wu ; Meiqing Zheng ; Shiqi Peng ; ^{sqpeng@bjmu.edu.cn}
• 关键词：Rabdosia rubescens ; Components ; Anti-inflammation activity ; Analgesic activity
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2011
• 出版时间：15 September, 2011
• 年：2011
• 卷：879
• 期：26
• 页码：2783-2793
• 全文大小：1K

文摘

In China the leaves of Rabdosia rubescens have been cooked in water and widely drank to treat inflammatory and pain related diseases. To explore the components that were possibly absorbed by people the aqueous extract of the leaves was prepared, and one single HPLC-PDA/(-)ESI-MS/MS analysis was developed to simultaneously determine the components. Using the HPLC-PDA analysis 39 peaks were found in the aqueous extract, while using the (-)ESI-MS/MS analysis we were able to identify 30 peaks represented components, including 5 nucleic acids, 21 phenolic acids and 4 diterpenoids. On mouse models the in vivo anti-inflammation and analgesic actions demonstrate that 0.32 g/kg of the aqueous extract of the leaves of Rabdosia rubescens can effectively inhibit the inflammation-induced chronic pain.