Experimental endometriosis reduction in rats treated with Uncaria tomentosa (cat's claw) extract

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• 作者：Joã ; o Nogueira Neto^a ; ^b ; ^{j.nogueira.n@uol.com.br} ; Tarc&#xed ; sio Mota Coelho^a ; Guilherme Carneiro Aguiar^a ; Laura Rosa Carvalho^a ; Ana Gisé ; lia Portela de Araú ; jo^a ; Manuel Joã ; o B.C. Girã ; o^b ; Eduardo Schor^b
• 关键词：Uncaria tomentosa ; Experimental endometriosis ; Drug therapy ; Wistar rats
• 刊名：European Journal of Obstetrics and Gynecology and Reproductive Biology
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：154
• 期：2
• 页码：205-208
• 全文大小：200 K

文摘

Objective

The aim of this study was to analyze the macroscopic and histological changes that occur in experimental endometriosis after treatment with Uncaria tomentosa.

Study design

Experimental endometriosis was induced in twenty-five female Wistar rats. After three weeks, 24 animals developed grade III experimental endometriosis and were divided into two groups. Group “U” received U. tomentosa extract orally (32 mg/day), and group “C” (control group) received a 0.9 % sodium chloride solution orally (1 ml/100 g of body weight/day). Both groups were treated with gavage for 14 days. At the surgical intervention and after the animal was euthanized, the implant volume was calculated with the following formula: [4π (length/2) × (width/2) × (height/2)/3]. The autotransplants were removed, dyed with hematoxylin–eosin, and analyzed by light microscopy. The Mann–Whitney test was used for the independent samples, and the Wilcoxon test analyzed the related samples, with a significance level of 5 % .

Results

The difference between the initial average volumes of the autotransplants was not significant between the groups (p = 0.18). However, the final average volumes were significantly different between the groups (p = 0.001). There was a significant increase (p = 0.01) between the initial and final average volumes in the control group, and treatment with the U. tomentosa caused a marked reduction in the growth over time (p = 0.009). Histologically, in the experimental group (n = 10) six rats had a well-preserved epithelial layer, three had mildly preserved epithelium, and one had poorly preserved epithelium. The epithelial layer occasionally presented sporadic epithelial cells. The control group (n = 12) presented seven cases (58.3 % ) of well-preserved epithelial cells and five cases (41.7 % ) of mildly preserved epithelial cells.

Conclusions

Cat's claw extract appears to be a promising alternative for treating endometriosis.