Cross-Regulation of C/EBPα and PPARγ Controls the Transcriptional Pathway of Adipogenesis and Insulin Sensitivity

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• 作者：Zhidan Wu ; Evan D Rosen ; Regina Brun ; Stefanie Hauser ; Guillaume Adelmant ; Amy E Troy ; Catherine McKeon ; Gretchen J Darlington ; Bruce M Spiegelman
• 刊名：Molecular Cell
• 出版年：1999
• 出版时间：February 1999
• 年：1999
• 卷：3
• 期：2
• 页码：151-158
• 全文大小：308 K

文摘

Mice deficient in C/EBPα have defective development of adipose tissue, but the precise role of C/EBPα has not been defined. Fibroblasts from C/EBPα(−/−) mice undergo adipose differentiation through expression and activation of PPARγ, though several clear defects are apparent. C/EBPα-deficient adipocytes accumulate less lipid, and they do not induce endogenous PPARγ, indicating that cross-regulation between C/EBPα and PPARγ is important in maintaining the differentiated state. The cells also show a complete absence of insulin-stimulated glucose transport, secondary to reduced gene expression and tyrosine phosphorylation for the insulin receptor and IRS-1. These results define multiple roles for C/EBPα in adipogenesis and show that cross-regulation between PPARγ and C/EBPα is a key component of the transcriptional control of this cell lineage.