Simultaneous Determination of Four Substances in Plasma and Dermal Microdialysates of Guinea Pig after Different Acupoints Administration of Fufang Baijiezi Gel

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• 作者：Guo-hua Wang ; Bao-xian Zhang ; ^{zhangbaoxian925@163.com" class="auth_mail" title="E-mail the corresponding author} ; Qi-xia Nie ; Chen Zang ; Qiong Zhu
• 关键词：acupoints administration ; Fufang Baijiezi Gel ; LC-MS ; microdialysis ; pharmacokinetics
• 刊名：Chinese Herbal Medicines
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：7
• 期：4
• 页码：365-370
• 全文大小：1293 K

文摘

To study the pharmacokinetic properties of Fufang Baijiezi Gel (FBG) after different acupoints administration.

Methods

a20">Sinapine thiocyanate, tetrahydro-palmatine, 6-gingerol, and asarinin, which were four substances of FBG, were determined by a sensitive liquid chromatography tandem mass spectrometry method (LC-MS) both in plasma and dermal microdialysates of guinea pig simultaneously. Microdialysates were separated on an Ultimate^® XB-Phenyl analytical column (150 mm × 2.1 mm, 5µm) and detected by electrospray ionization (ESI) in selected ion monitoring (SIM) mode. The method was validated in terms of selectivity, linearity, sensitivity, and recovery. Result A significant difference was observed in main pharmacokinetic parameters of C_max, t_max, and AUC between acupoints administration and nonacupoints administration.

Conclusion

Acupoints administration resulted in a more obvious increase in bioavailability of sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin than nonacupoints administration.