Novel process for producing 6-deoxy monosaccharides from l-fucose by coupling and sequential enzymatic method

详细信息    查看全文

• 作者：Sirinan Shompoosang¹ ; ² ; ³ ; Akihide Yoshihara² ; Keiko Uechi¹ ; ² ; Yasuhiko Asada⁴ ; Kenji Morimoto² ; ^{morimoto@ag.kagawa-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：l-Fucose ; 6-Deoxy-l-talose ; 6-Deoxy-l-sorbose ; 6-Deoxy-l-gulose ; 6-Deoxy-l-idose ; d-Arabinose isomerase ; l-Rhamnose isomerase ; d-Allulose 3-epimerase ; l-Ribose isomerase ; d-Glucose isomerase
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：121
• 期：1
• 页码：1-6
• 全文大小：641 K

文摘

We biosynthesized 6-deoxy-l-talose, 6-deoxy-l-sorbose, 6-deoxy-l-gulose, and 6-deoxy-l-idose, which rarely exist in nature, from l-fucose by coupling and sequential enzymatic reactions. The first product, 6-deoxy-l-talose, was directly produced from l-fucose by the coupling reactions of immobilized d-arabinose isomerase and immobilized l-rhamnose isomerase. In one-pot reactions, the equilibrium ratio of l-fucose, l-fuculose, and 6-deoxy-l-talose was 80:9:11. In contrast, 6-deoxy-l-sorbose, 6-deoxy-l-gulose, and 6-deoxy-l-idose were produced from l-fucose by sequential enzymatic reactions. d-Arabinose isomerase converted l-fucose into l-fuculose with a ratio of 88:12. Purified l-fuculose was further epimerized into 6-deoxy-l-sorbose by d-allulose 3-epimerase with a ratio of 40:60. Finally, purified 6-deoxy-l-sorbose was isomerized into both 6-deoxy-l-gulose with an equilibrium ratio of 40:60 by l-ribose isomerase, and 6-deoxy-l-idose with an equilibrium ratio of 73:27 by d-glucose isomerase. Based on the amount of l-fucose used, the production yields of 6-deoxy-l-talose, 6-deoxy-l-sorbose, 6-deoxy-l-gulose, and 6-deoxy-l-idose were 7.1%, 14%, 2%, and 2.4%, respectively.