Single-step bioconversion for the preparation of l-gulose and l-galactose
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- 作者：Ryan D. Woodyer ; Trevor N. Christ ; Kara A. Deweese
- 关键词：l-Gulose ; l-Galactose ; Directed evolution ; Phosphotransferase system ; Mannitol-1-dehydrogenase
- 刊名：Carbohydrate Research
- 出版年：2010
- 出版时间：11 February 2010
- 年：2010
- 卷：345
- 期：3
- 页码：363-368
- 全文大小：419 K
文摘
Both carbohydrate monomers l-gulose and l-galactose are rarely found in nature, but are of great importance in pharmacy R&D and manufacturing. A method for the production of l-gulose and l-galactose is described that utilizes recombinant Escherichia coli harboring a unique mannitol dehydrogenase. The recombinant E. coli system was optimized by genetic manipulation and directed evolution of the recombinant protein to improve conversion. The resulting production process requires a single step, represents the first readily scalable system for the production of these sugars, is environmentally friendly, and utilizes inexpensive reagents, while producing l-galactose at 4.6 g L−1 d−1 and l-gulose at 0.90 g L−1 d−1.