Expression, purification, and characterization of soluble and active glutamate-specific endopeptidase in Bacillus licheniformis and Pichia pastoris
详细信息 查看全文
- 作者：Wei Ye ; Taomei Liu ; Weimin Zhang ; wmzhang@gdim.cn" class="auth_mail" title="E-mail the corresponding author ; Guohui Tan ; Zhanghua Sun ; Haohua Li
- 关键词：Glutamate-specific endopeptidase ; Maturation ; Enzymatic kinetics ; Bacillus licheniformis ; Pichia pastoris
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：132
- 期：Complete
- 页码：24-30
- 全文大小：1174 K
文摘
- •
Soluble and enzymatically active GSE-BLs were firstly expressed in B. licheniformis and P. pastoris.
- •
Pure and active GSE-BL-B and GSE-BL-P were obtained after one-step His6 tag affinity chromatography.
- •
Full length GSE-BL-P showed enzymatic activity because of glycolysation.
- •
Mature GSE-BL-B and GSE-BL-P showed similar enzymatic kinetics as GSE-BL-E.