A highly sensitive near-infrared ratiometric fluorescent probe for detecting nitroreductase and cellular imaging

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• 作者：Dongjian Zhu^a ; ^bAuthor Vitae ; Lin Xue^bAuthor Vitae ; Guoping Li^bAuthor Vitae ; Hua Jiang^a ; ^{jiangh@bnu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Fluorescent probe ; Near-infrared ; Ratiometric ; Nitroreductase ; Cellular imaging
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：222
• 期：Complete
• 页码：419-424
• 全文大小：1503 K

文摘

Based on the selective reduction of nitro group to hydroxylamine or amino group by nitroreductase in the presence of NADPH as an electron donor, a near-infrared ratiometric fluorescent probe CyNNO₂ was designed and synthesized. CyNNO₂ containing a p-nitrobenzyl moiety as a reactive group can selectively respond to nitroreductase with a ratiometric fluorescence signal output. The limit of detection for nitroreductase is 0.0058 ng/mL. Moreover, the probe can be used to image the endogenous nitroreductase in A549 cells under hypoxic condition.