Improving the power to detect differentially expressed genes in comparative microarray experiments by including information from self–self hybridizations

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• 作者：Arief Gusnanto ; Brian Tom ; Philippa Burns ; Iain Macaulay ; Daphne C. Thijssen-Timmer ; Marloes R. Tijssen ; Cordelia Langford ; Nicholas Watkins ; Willem Ouweh ; Carlo Berzuini ; et al.
• 关键词：Linear models ; Self– ; self hybridizations ; Differentially expressed ; Gene expressions ; Microarray
• 刊名：Computational Biology and Chemistry
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：31
• 期：3
• 页码：178-185
• 全文大小：327 K

文摘

Our ability to detect differentially expressed genes in a microarray experiment can be hampered when the number of biological samples of interest is limited. In this situation, we propose the use of information from self–self hybridizations to acuminate our inference of differential expression. A unified modelling strategy is developed to allow better estimation of the error variance. This principle is similar to the use of a pooled variance estimate in the two-sample t-test. The results from real dataset examples suggest that we can detect more genes that are differentially expressed in the combined models. Our simulation study provides evidence that this method increases sensitivity compared to using the information from comparative hybridizations alone, given the same control for false discovery rate. The largest increase in sensitivity occurs when the amount of information in the comparative hybridization is limited.