Evidence Supporting a Protective Role for Th9 and Th22 Cytokines in Human and Experimental Periapical Lesions

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• 作者：Andreza Maria Fabio Aranha ; DDS ; MSc ; PhD^&lowast ; ; ^&dagger ; ; Carlos Eduardo Repeke ; DDS ; MSc^&lowast ; ; Thiago Pompermaier Garlet ; DDS ; MSc ; PhD^&Dagger ; ; Andreia Espindola Vieira ; DDS ; MSc^&lowast ; ; Ana Paula Campanelli ; MSc ; PhD^&lowast ; ; Ana Paula Favaro Trombone ; PharmD ; MSc ; PhD^&lowast ; ; Ariadne Letra ; DDS ; MSc ; PhD^§ ; ; Renato Menezes Silva ; DDS ; MSc ; PhD^§ ; ; Gustavo Pompermaier Garlet ; DDS ; MSc ; PhD^&lowast ; ; ^{garletgp@usp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Apical granulomas ; cytokines ; immunoregulation ; qPCR
• 刊名：Journal of Endodontics
• 出版年：2013
• 出版时间：January 2013
• 年：2013
• 卷：39
• 期：1
• 页码：83-87
• 全文大小：282 K

文摘

The development of periapical granulomas is dependent on the host response and involves Th1, Th2, Th17, and Treg-related cytokines. The discovery of new Th9 and Th22 subsets, with important immunomodulatory roles mediated by interleukin (IL)-9 and IL-22, respectively, emphasizes the need for reevaluation of current cytokine paradigms in context of periapical lesions. We investigated the expression of IL-9 and IL-22 in active and stable human granulomas and throughout experimental lesion development in mice.

Methods

Periapical granulomas (N = 83) and control specimens (N = 24) were evaluated regarding the expression of IL-9 and IL-22 via real-time polymerase chain reaction. Experimental periapical lesions were induced in mice (pulp exposure and bacterial inoculation) and the lesions evolution correlation with IL-9 and IL-22 expression kinetics was evaluated.

Results

IL-9 and IL-22 mRNA expression was higher in periapical lesions than in control samples; higher levels of IL-9 and IL-22 were observed in inactive than in active lesions. In the experimental lesions model, increasing levels of IL-9 and IL-22 mRNA were detected in the lesions, and inverse correlations were found between IL-9 and IL-22 and the increase of lesion area in the different time point intervals.

Conclusions

Our results suggest that Th9 and Th22 pathways may contribute to human and experimental periapical lesion stability.