Site-Specific Serine Phosphorylation of the IL-3 Receptor Is Required for Hemopoietic Cell Survival

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• 作者：Guthridge ; Mark A. ; Stomski ; Frank C. ; Barry ; Emma F. ; Winnall ; Wendy ; Woodcock ; Joanna M. ; et. al.
• 刊名：Molecular Cell
• 出版年：2000
• 出版时间：July, 2000
• 年：2000
• 卷：6
• 期：1
• 页码：99-108
• 全文大小：456 K

文摘

In the hemopoietic compartment, IL-3, GM-CSF, and IL-5 receptors are major transducers of survival signals; however, the receptor-proximal events that determine this vital function have not been defined. We have found that IL-3 stimulation induces phosphorylation of Ser-585 of β_c. This promotes the association of phospho-Ser-585 of β_c with 14-3-3 and the p85 subunit of PI 3-K. Mutation of Ser-585 specifically impairs the PI 3-K signaling pathway and reduces cell survival in response to IL-3. These results define a distinct IL-3 receptor–mediated survival pathway regulated by site-specific receptor serine phosphorylation and 14-3-3 binding and suggest that this novel mode of signaling may be utilized by disparate transmembrane receptors that have as a common theme the transduction of survival signals.