Inhibition of salivary and pancreatic 伪-amylases by a pinh茫o coat (Araucaria angustifolia) extract rich in condensed tannin

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• 作者：Simone Mariano da Silva ; Elo谩 Ang茅lica Koehnlein ; Adelar Bracht ; Rafael Castoldi ; Gutierrez Rodrigues de Morais ; Mauro Luciano Baesso ; Rosely Aparecida Peralta ; Cristina Giatti Marques de Souza ; Anacharis Babeto de S谩-Nakanishi ; Rosane Marina Peralta
• 关键词：Araucaria angustifolia ; Amylase inhibitors ; Condensed tannins ; Diabetes ; Pinh&atilde ; o
• 刊名：Food Research International
• 出版年：February, 2014
• 年：2014
• 卷：56
• 期：Complete
• 页码：1-8
• 全文大小：487 K

文摘

The purpose of the present work was to investigate the possible inhibitory effect of a pinh茫o coat extract rich in condensed tannin on the activity of 伪-amylases (human salivary and porcine pancreatic). Experiments with the classic 伪-amylase inhibitor acarbose and a condensed tannin from Acacia mearnsii were done for comparative purposes. Fourier transform-infrared spectroscopy analysis of the pinh茫o coat tannin revealed a higher proportion of procyanidins to prodelphinidins when compared to the A. mearnsii tannin. The pinh茫o coat extract rich in condensed tannin was an effective inhibitor of both human salivary and porcine pancreatic 伪-amylase. Inhibition was of the mixed S-parabolic I-parabolic type. For the human salivary 伪-amylase the inhibition constants were 56.88 卤 5.74 and 103.27 卤 11.85 渭g/L; for the porcine pancreatic 伪-amylase the inhibition constants were smaller, namely, 20.25 卤 1.97 and 46.79 卤 4.57 渭g/L. The decreasing potency sequence was: acarbose > pinh茫o coat extract rich in tannin > A. mearnsii tannin. Similarly to acarbose and the A. mearnsii tannin, the pinh茫o coat extract was also effective in diminishing the post-prandial glycemic levels in rats after starch administration. The inhibitory properties of the pinh茫o coat extract indicate that it can be used to suppress postprandial hyperglycemia in diabetic patients.