Induction of mitotic gynogenesis in turbot Scophthalmus maximus
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- 作者：Zhen Menga ; b ; Xinfu Liub ; ; Bin Liub ; Peng Hua ; Yudong Jiab ; Zhi Yangc ; Hesen Zhangd ; Xuezhou Liub ; Jilin Leib ; leijl@ysfri.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Scophthalmus maximus ; Mitotic gynogenesis ; Sex determination mechanism ; Cryopreserved sperm ; Pagrus major
- 刊名：Aquaculture
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：451
- 期：Complete
- 页码：429-435
- 全文大小：536 K
文摘
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Artificial mitotic gynogenesis in turbot was induced by hydrostatic pressure shock of eggs activated by heterologous sperm.
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The optimal parameters of mitotic gynogenesis were determined to be initiated at 85–90 maf with 75 MPa for 6 min.
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A mitotic gynogen of more than 7000 normal larvae was produced and, at 150 dah, a total of 124 juveniles survived.
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The ploidy of survived juveniles was confirmed to be diploid by flow cytometry and their sex ratio was 1:1 female to male.
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The assumption of a genetic mechanism of sex determination of female heterogametes (ZW/ZZ) in turbot is supported.