Suppression of reactive oxygen species by glyceraldehyde-3-phosphate dehydrogenase

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• 作者：Dongwon Baek ; Yinhua Jin ; Jae Cheol Jeong ; Hyo-Jung Lee ; Haejeong Moon ; Jiyoung Lee ; Dongjin Shin ; Chang Ho Kang ; Doh Hoon Kim ; Jaesung Nam ; Sang Yeol Lee ; Dae-Jin Yun
• 关键词：Arabidopsis thaliana ; Cruciferae ; Saccharomyces cerevisiae ; Glyceraldehyde-3-phosphate dehydrogenase ; Bax-induced cell death ; Reactive oxygen species ; Multifunctional protein
• 刊名：Phytochemistry
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：69
• 期：2
• 页码：333-338
• 全文大小：931 K

文摘

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classical glycolytic enzyme, is involved in cellular energy production and has important housekeeping functions. In this report, we show that a GAPDH from Arabidopsis, GAPDHa, has a novel function involved in H₂O₂-mediated cell death in yeast and Arabidopsis protoplasts. GAPDHa was cloned along with other plant genes that suppress Bax-induced cell death in yeast. Flow cytometry analyses with dihydrorhodamine 123 indicated that H₂O₂ production mediated by Bax expression in yeast cells was greatly reduced when Bax was coexpressed with GAPDHa. In plants, GAPDHa transcript levels were greatly increased by H₂O₂ treatment. Furthermore, transformation of GAPDHa into Arabidopsis protoplasts strongly suppressed heat shock-induced H₂O₂ production and cell death. Together, our results indicate that GAPDH controls generation of H₂O₂ by Bax and heat shock, which in turn suppresses cell death in yeast and plant cells.