X-gal® inhibits the swarming of Vibrio species
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- 作者：Moon-Young Kim ; Ra-Young Park ; Young-Hoon Bai ; Yoon-Young Chung ; Choon-Mee Kim ; Soo-Young Kim ; Joon-Haeng Rhee and Sung-Heui Shin
- 关键词：Vibrio ; X-gal ; β ; -galactosidase ; Swarming
- 刊名：Journal of Microbiological Methods
- 出版年：2006
- 出版时间：September 2006
- 年：2006
- 卷：66
- 期：3
- 页码：552-555
- 全文大小：206 K
文摘
The expressional levels of genes in swarmer cells can be determined by a simple method using X-gal-containing semisolid agars and lacZ-fusion transcription reporter strains of the genes concerned. However, X-gal alone inhibited the swarming of Vibrio, regardless of their ability to digest X-gal. Moreover, X-gal inhibited the growth of V. vulnificus containing functional lacZ. These effects of X-gal itself should be carefully considered when trying to determine the expression levels of genes in swarming cells using X-gal-containing semisolid agar.